Methotrexate (MTX)


Risk factors for methotrexate hepatotoxicity include:


Mechanism of action: not fully understood


Methotrexate inhibits dihydrofolate reductase, inhibiting purine synthesis, thereby inhibiting DNA and RNA replication

Methotrexate alters multiple cellular pathways, including increased adenosine signaling as well as down-regulation of cytokines, eicosanoids, and adhesion molecules.

Methotrexate is metabolized by the liver (first-pass hepatic metabolism) and about 10% is excreted in bile.

After methotrexate enters peripheral blood cells, it undergoes polyglutamylation, (varying chain lengths of glutamate metabolites are made). The enzymatic processes of these glutamate metabolites contribute to the steady state of the drug